Simian rotavirus SA11 replication in cell cultures

Effect of actinomycin D on simian rotavirus (SA11) replication in cell culture.

Rotaviruses are the major cause of viral diarrhea in humans and animals. Actinomycin D (Act D) is an antibiotic that intercalates DNA and therefore inhibits DNA-dependent transcription. The current study was carried out to assess the influence of Act D on the replication of simian rotavirus (SA11) in cell culture. Virus-infected MA-104 cell cultures were studied in the presence of Act D at conc...

Preferential Assortment of Rearranged NSP3 Gene in the Genetic Background of Simian Rotavirus SA11

Selective assortment of rotavirus NSP3 gene in the genetic background of simian rotavirus strain SA11 was analyzed by using single-NSP3 gene-substitution reassortants having NSP3 gene from human rotavirus strain KU (KU-R) or porcine rotavirus strain OSU (OSU-R) in the SA11 background, and an SA11-derived variant Ga613 having a rearranged SA11NSP3 gene which is 937 nucleotides longer than the or...

Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence.

DNA sequences complementary to the double-stranded RNA coding for the neutralization antigen (glycoprotein VP7) of simian rotavirus SA11 have been cloned. The VP7 gene consists of 1,062 nucleotides, containing an uninterrupted coding sequence of 978 nucleotides which specifies a glycoprotein of 326 amino acids. The significance of a second possible initiation site 30 nucleotides downstream from...

Studies on the replication of African Horse Sickness Virus in two different cell line cultures

Commercial simian virus antisera that inhibit virus replication in primary monkey kidney cell cultures.

The incorporation of hyperimmune serum into cell culture medium to control endogenous viral infections of primary cells can have a significant effect on the replication of other viruses. When commercial simian virus 5 or simian virus 40 antiserum was used with primary monkey kidney cell cultures, we found a significant inhibition (greater than 90%) of the replication of parainfluenza virus type...